Journal: PLoS ONE

Article Title: Genetic Ablation of Afadin Causes Mislocalization and Deformation of Paneth Cells in the Mouse Small Intestinal Epithelium

doi: 10.1371/journal.pone.0110549

Figure Lengend Snippet: ( A ) Western blotting of afadin and EphB3 using immunoprecipitates with the indicated antibodies from the Ls174T colon cancer cell line. ( B ) Immunostaining of the small intestine of control and afadin -cKO mice with antibodies against EphB3 (green), lysozyme (red), and Hoechst33258 (blue). Scale bars = 50 µm. ( C ) Relative intensity of EphB3 signal in crypt cells to that of non-crypt cells is shown. 18 crypts from 6 pictures (control) and 24 crypts from 8 pictures ( afadin -cKO) were analyzed.

Article Snippet: The colon cancer cell line Ls174T (DS Pharma Biomedical Co., Osaka, Japan) was cultured in MEM containing 1% NEAA, 2 mM L-glutamine, and 10% FBS and lysed in 50 mM Tris HCl (pH 7.5), 150 mM NaCl, 1 mM MgCl 2 , 1% Nonidet P-40, 1 mM EGTA, and 10% glycerol supplemented with 1 μg/ml aprotinin, 1 μg/ml leupeptin, 20 μg/ml phenylmethylsulfonyl fluoride, and phosphatase inhibitors.

Techniques: Western Blot, Immunostaining, Control

Journal: Current Issues in Molecular Biology

Article Title: Phage Display Reveals VLRB-Mediated Recognition of Minimal Tumor Glycan Antigen Sialyl-Tn

doi: 10.3390/cimb47100802

Figure Lengend Snippet: FITC-stained and composite fluorescence images (400× magnification) of carcinoma-derived cell lines ( a ) HeLa and ( b ) LS174T expressing the sTn antigen as detected by ccombodies A8 and B11. Cell nuclei were counterstained with Hoechst for reference. ( c ) HEK293F cell line and an unrelated ccombody (UC) were used as negative controls, while VVL served as a positive control. The red scale bar represents 10 µm.

Article Snippet: Sang Soo Kang of the College of Medicine, Gyeongsang National University, Jinju, Republic of Korea), LS174T (Korean Cell Line Bank, Seoul, Republic of Korea), and HEK293F (ThermoFisher Scientific, Waltham, MA, USA).

Techniques: Staining, Fluorescence, Derivative Assay, Expressing, Positive Control